What is cryopreservation?
Cryopreservation is a long-term storage technique with very low temperatures used to preserve the structurally intact living cells and tissues for an extended period of time, at a relatively low cost.
The standard procedure for cryopreservation is to freeze cells slowly until they reach an average temperature below -70°C through the use of a cryoprotectant. Then vials are transferred to a liquid-nitrogen freezer to maintain them at temperatures below -130°C.
There are two basic types of liquid nitrogen storage systems:
- Immersing vials in the liquid
- Holding vials in the vapor phase above the liquid